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Aspergillus niger library (LMBP LIB9)
Library name: | Aspergillus niger library |
Library type: | cDNA |
Library accession number: | LMBP LIB9 |
Organism: | Aspergillus niger |
Priming method: | NotI-(dT)15 (unidirectional) |
Vector name: | pSCGAL10-SN |
Vector sequence file: | p2471.txt |
Vector circular map: | p2471.pdf |
Vector accession number: | LMBP 2471 |
Vector type: | yeast expression vector |
Cloning sites: | SfiI-NotI |
Library coverage: | 2,0 x 10E5 |
Average insert size: | 0,60 kb |
Distributed as: | pooled library DNA |
DNA concentration: | 150 ng/µl |
Volume: | 5 µl |
Further information: | Aspergillus niger N402 was grown in rich YPD medium, consisting of 1% bacto-yeast extract, 2% bacto-peptone and 2% dextrose. The host strain Escherichia coli K12 MC1061 or DH5α can be used for electroporation to amplify the library. The transformation mixture can then be streaked on selective solid medium (LB-Lennox + 100 µg/ml ampicillin) and cultivated at 37°C to obtain the different library clones. |
History of deposit: | The cDNA library was deposited by Prof. R. Contreras1. 1 Department of Molecular Biology, Ghent University, Belgium |
Library reference: | Veldhuisen et al., Mol. Gen. Genet. 256 (1997), 446-455 [PMID: 9393442] |
Related reference: | - |
Restricted distribution: | - BCCM MTA |
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